Effect of manuronic acid oligosaccharide on aging cardiomyocytes induced by H2O2

The Mannuronic oligosaccharides  oligosaccharide is a kind of algin oligosaccharide, which is processed by algin and has various biological activities such as anti-oxidation, anti-inflammation and anti-tumor, and has important clinical application value. In this experiment, the model of myocardial cell senescence was established by H2O2 induction, To investigate the protective effect of Mannuronic oligosaccharides on aging cardiomyocytes.

Five groups were divided into five groups: control group (a group), H2O2 group (b group), H2O2 + Mannuronic oligosaccharide (m3k) low dose group (c group), H2O2 group + manuronic oligosaccharide (M3K) middle dose group (d group), H2O2 + manuronic oligosaccharide (M3K) high dose group (e group). When the cell fusion level was up to 50%, the cells of group b, c, d and e were induced to establish the aging model by adding 100μmol/L / l of H2O2, and the fresh DMEM was replaced after 4 h. H2O2 group continued to culture for 48 hours, Mannuronic oligosaccharide (M3K) was added to each group for 48 hours, and 50, 100mg Mannuronic oligosaccharide (M3K) was added to each group for 48 hours. The number of cardiomyocytes was detected, the expression of P21 and P53 protein in cardiomyocytes was compared, and the expression of beclin1,Atg7 and LC3- II mRNA in each group was compared. The results showed that after 48 hours of culture, the number of cells in group B (H2O2 group) was significantly lower than that in control group, and the number of cells in group C, D and E increased in a dose-dependent manner compared with group H2O2, and the number of cells in group C, group D and group E was 51.3%, 82.2% and 97.4%, respectively. Compared with the control group, the expression of P21 and P53 protein in group B (H2O2 group) increased significantly, and after 48 hours of mannose oligosaccharide (M3K) intervention, the expression of P21 and P53 protein decreased with the increase of Mannuronic oligosaccharide (M3K) concentration, and the expression ratio of P21 protein in group C, group D and group E was 20.0%, 29.3% and 40.3% lower than that in group b, respectively, and the expression of p21 protein in group C, D and E was 20.0%, 29.3% and 40.3%, respectively. The P53 protein ratio decreased by 30.2%,51.2%, and 62.2%, respectively, compared with group B. compared with the control group, the expression levels of beclin1, Atg7 and LC3- II mRNA of H2O2 group decreased significantly. After 48 hours of Mannuronic oligosaccharide (M3K) intervention, the expression of beclin1, Atg7 and LC3- II mRNA in cardiomyocytes of each group increased in a concentration-dependent manner. The expression of beclin1 in group C, group D and group E was 35.9%, 78.0%, 132.6% and 96.4%, respectively, which was 18.7%, 45.3% and 90.4% higher than that in group b, respectively. the expression of mrna in group C, D and group E increased by 62.6%, 96.4% and 18.7%, 45.3% and 90.4%, respectively. compared with group b, the expression of mRNA in group C, group D and group E increased by 35.9%, 73.0% and 90.4%, respectively.


The results showed that H2O2 induced intracellular oxidative stress and cell senescence, which showed cell cycle arrest and decreased proliferation. Mannuronic oligosaccharide (m3k) could down-regulate the expression of p21 and p53 protein in aging cells for 48 hours, alleviate the cell cycle arrest, accelerate the proliferation of aging cardiomyocytes, and thus delay the senescence of cardiomyocytes. After treatment with Mannuronic oligosaccharide (m3k), the expression of autophagy related genes beclin1, Atg7 and LC3- II mRNA increased, indicating that Mannuronic oligosaccharide (m3k) may be related to autophagy activation.

Leave a Comment
Your email address will not be published. Required fields are marked *
Submit Comment
People also viewed Of
Mechanism of antiviral action and structure-activity relationship of Marine polysaccharide sulfate Application of Chitosan Flocculant in Purification of Traditional Chinese Medicine Application of Chitosan in Food Brown algae oligosaccharide improves drought resistance of wheat Production Process of Chitosan Oligosaccharide The difference between Chitin, Chitosan and Chitosan oligosaccharide Good news!Our marine oligosaccharide standard products won the national standard sample certification! Application of chitosan oligosaccharide in potato cultivation Application of Chitosan oligosaccharide in agriculture--Regulate defense responses of plants Chitosan Oligosaccharide can prolong fruit storage Application of chitosan oligosaccharide in improving fruit quality Application of COS in seed soaking Application of COS in promoting seedling growth Effect of Foliar Spraying of Chitosan Oligosaccharide on Growth and Yield of Chinese Cabbage Chitotriose Trihydrochloride Effects of chitosan oligosaccharides on cold resistance of plants A new environmental protection plant regulator--Chitosan oligosaccharide Carrageenan oligosaccharide with antiviral activity The prebiotic effect of Neoagarooligosaccharides Antioxidant activity of polymannodic and polygulonic Application of Chitooligosaccharides in Aquaculture Bioactivity of chitosan oligosaccharides with different polymerization degrees Immunomodulatory effect of mannuronic oligosaccharides Alginate oligosaccharide elicitor induces tobacco resistance to low temperature Effect of Chitooligosaccharides on Cotton Growth and Yield Effects of alginate oligosaccharides on germination and physiological characteristics of barley seeds Effect of Chitooligosaccharide Seed Dressing on the Germination of Flax Seeds Effect of Chitooligosaccharides on the Fresh-keeping Effect of Chilled Beef Effect of mannuronic oligosaccharides on H2O2 induced senescence of cardiomyocytes Induction of Agaro-oligosaccharide on the resistance of Porphyra haitanensis Effect of Chitooligosaccharides of Different Molecular Weight on Cold Resistance of Cucumber Plug Seedlings Effects of Chitooligosaccharides on Pepper Seed Germination and Seedling Antioxidant Enzyme Activities Study on the effect of oligochitosan on the cold resistance of eggplant seedlings Effect of Chito-oligosaccharide Coated Paper Packaging on Postharvest Freshness of Broccoli Effect of Alginate Oligosaccharide on Soybean Seed Germination and Seedling Physiology Effects of Alginate Oligosaccharides on Soybean Seed Germination and Seedling Physiology Effect of Alginate Oligosaccharide on Sorghum Seed Germination and Seedling Physiological Characteristics Inhibition of water-soluble chitosan on several common bacteria Effect of Agar Oligosaccharide on Cucumber Resistance and Acetamiprid Residue Study on the anti-obesity activity of oligochitosan Study on the Application Effect of Alginate Oligosaccharide in Agriculture Research and Application of Chitooligosaccharides in Fish Feed
People who viewed this item also viewed
Concact Us Now
Chitosan Oligosaccharide, Alginate Oligosaccharide, Chitosan Oligosaccharide Suppliers - BZ Oligo
Headquarter:Room 806, D&D Fortune Center, No. 182-6 Haier Road, Qingdao 266061, China
You can trust us
We are a professional Manufacturer in China, and we are constantly innovating so that our customers can have better products and services.
© 2018 Gobalsir Inc.    RELATED ARTICLES    SiteMap.html    SiteMap.xml    Terms of Service      Privacy Policy
Marketing Support by Globalsir
Enter your inquiry details, We will reply you in 24 hours.
Name can't be empty
E-mail can't be empty
Company can't be empty
Phone can't be empty
Products can't be empty
Message can't be empty
Verification code error