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Effect of mannuronic oligosaccharides on H2O2 induced senescence of cardiomyocytes

Mannuronic oligosaccharides is a kind of alginate oligosaccharides, which is processed from alginate. It has many biological activities, such as antioxidant, anti-inflammatory, anti-tumor and so on. It has important clinical application value.
 
Mou Jie, et al. From Department of Geriatrics, Affiliated Hospital of Qingdao University, used 100umol / L H2O2 to construct H9c2 cardiomyocyte aging model, and used different concentrations (10, 50, 100 mg / ml) of m3k to intervene aging cardiomyocytes to observe the effect of m3k on cardiomyocyte aging. The experiment was divided into control group (a), H2O2 group (b), H2O2 + mannuronic oligosaccharides (m3k) low dose group (c), H2O2 + mannuronic oligosaccharides (m3k) medium dose group (d), H2O2 + mannuronic oligosaccharides (m3k) high dose group (E). The number of cardiomyocytes in each group was observed by inverted microscope. The expressions of p21 and p53 were detected by Western blot (Table 1). The mRNA expressions of Beclin1, Atg7 and LC3 - Ⅱ were detected by pt-prc (Table 2). The results showed that H2O2 induced oxidative stress in cardiomyocytes and induced cell senescence. After senescence, cell cycle arrest and proliferation decreased. Mannuronic oligosaccharides (m3k) can down regulate the expression of p21 and p53 protein, alleviate the cell cycle arrest and accelerate the proliferation of senescent cardiomyocytes, and then delay the senescence of cardiomyocytes. The mRNA expression of autophagy related genes Beclin1, Atg7 and LC3 - Ⅱ increased after treatment with mannuronic oligosaccharides (m3k), suggesting that the anti-aging effect of mannuronic oligosaccharides (m3k) may be related to the activation of autophagy.
 
Table 1 Comparison of the number of cardiomyocytes and the expression of p21 and p53 protein in each group (n = 3, X ± s)
 
Table 2 Comparison of mRNA expression of Beclin1, Atg7 and LC3 - Ⅱ in cardiomyocytes of each group (n = 3, X ± s)
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