Effect of Alginate Oligosaccharide on Soybean Seed Germination and Seedling Physiology

Alginate Oligosaccharide is a marine oligosaccharide that is degraded by biological enzymes and chemical oxidation of alginate. It has significant anti-tumor, nerve repair, immune enhancement, growth promotion and many other physiological activities. Zhang Shoudong and others studied the effects of different mass concentrations on soybean seed germination and seedling physiology.

The distilled water used in the experiment was prepared into 250 mL of Alginate Oligosaccharide solution with mass concentration of 0.625, 1.25, 2.5, and 5g/L. After disinfecting the full and uniform soybean seeds, they were placed in an oligosaccharide solution at room temperature for 12 hours, treated with distilled water as a control, 100 seeds per group, and 3 parallel groups for each treatment. After the soaked and expanded seeds were washed with distilled water, they were placed in a paper bed in a thermostat at 25° C. to germinate and cultivated. The germination rate was measured on the 5th and 8th days. After the treated seeds were soaked for 12 h, the lipase activity was measured every 2 days. Select the seeds that have germinated and grow in a similar manner, sow them in pots, and cultivate them under light at room temperature. After 24 days, the growth and various physiological indexes of soybean seedlings were measured, and each treatment was repeated 5 times. The test results showed that: 0.625g/L Alginate Oligosaccharide can improve the germination vigor of soybean seeds (Figure 1) and lipase activity during germination (Figure 2), and it can also increase the mass fraction of chloroplast pigment and biomass of seedlings (Table 1). Overall, the effect of Alginate Oligosaccharide on the mass fraction of soluble protein is not significant. With the increase in the concentration of Alginate Oligosaccharide, the root vitality gradually increased. It shows that soaking seeds with low mass concentration of Alginate Oligosaccharide can increase the germination rate of soybean seeds, the activity of lipase during germination and the physiological activity of seedlings, speed up the metabolic process, promote soybean growth, and high mass concentration of Alginate Oligosaccharide inhibit soybean growth, but the root vitality will be Compensation is enhanced to ensure the normal growth of plants.

Figure 1 Germination potential of soybean seeds soaked under different mass concentrations of Alginate Oligosaccharide

Figure 2 Lipase activity of soybean seeds soaked with different mass concentrations of Alginate Oligosaccharide

Table 1 The chlorophyll content, soluble protein content and root vitality of soybean leaves soaked with different mass concentrations of Alginate Oligosaccharide

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