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Effects of Chitooligosaccharides on Pepper Seed Germination and Seedling Antioxidant Enzyme Activities

Chitooligosaccharides have good biological activity, can regulate plant growth, increase the yield of crops and fruits and vegetables, so the application in agriculture is increasing day by day. The application in agriculture includes promoting seed germination and plant growth. In this paper, Lu Jianling and others selected pepper seeds as the research object and discussed the effects of different concentrations of chitooligosaccharides on the germination of pepper seeds.

The experiment selects pepper seeds with full grains and the same size, rinses them, and uses distilled water (CK) and 4 different mass concentrations of 0.01(A), 0.10(B), 1.00(C), 100.00(D)mg at room temperature. /ml chitosan oligosaccharide solution soaking seeds for 20h, each process 100 grains, 3 times. Rinse the seeds with distilled water 3 times after soaking, sow them in a petri dish covered with 2 layers of filter paper, add 10ml of distilled water to infiltrate, cover the seeds with a layer of filter paper, and place them in a (29 ± 1) ℃ constant temperature box in the dark environment for sprouting and sprouting During this period, add proper amount of distilled water to each treatment every day. Observe and record the germination-related indicators every day. After 6 days of accelerating germination, the seed germination vigor was measured, and the germination rate was measured on the 12th day, and the vigor index was calculated. Randomly select seedlings cultivated to 12 days using the method of superoxide dismutase (SOD) test kit for superoxide dismutase (SOD) activity. The results of the study showed (Table 1, Figures 1 and 2) a certain concentration of oligochitosan can promote seed germination, and the effect of 0.10 mg/L oligochitosan is the most significant; different concentrations of oligochitosan can activate the antioxidant of pepper seedlings Enzyme activity.

Table 1 Effects of different concentrations of oligochitosan on the germination of pepper seeds

Figure 1 The effect of different concentrations of oligochitosan on SOD activity

Figure 2 The effect of different concentrations of oligochitosan on the activity of CAT

Qingdao HEHAI specializes in the production of chitosan oligosaccharides. If you have any needs, please email to june@marine-oligo.com

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